MicroRNAs are foreseen as promising biomarkers for diagnosis, prognosis and follow up of cancer diseases. First things first: be able to detect these biomarkers reliably and sensitively. The objective of this work is to propose a novel technology for the accurate quantification of these microRNA molecules, combining DNA nanotechnology and droplet microfluidics.
I am Guillaume, postdoc fellow at ESPCI Paris in the Laboratory Gulliver located in Paris.
My research interests are centered around DNA nanotechnologies and molecular programming, from their fundamental demonstrations to applications in diagnostics and biotechnology. My work is a lot inspired by cellular and system biology: a single cell is an incredible object that orchestrates hundreds of entangled signaling pathways to control homeostasis, energy production, genome integrity, division… Using a bottom-up approach, we rationally build artificial DNA/enzyme-based reaction networks that emulate cellular functionalities: differentiation from multistable systems, excitability akin to neuron firing or intercellular communication mimicked with DNA-programmed particles. Building on this knowledge and expertise, we develop technologies for molecular markers quantification, in particular microRNAs that gain a lot of attraction as cancer or neurodegenerative biomarkers owing to their central role in the regulation of gene expression. Combining molecular programming with microfluidics, we recently developed an isothermal droplet digital detection assay for the accurate quantification of microRNA biomarkers.
The team is also interested in engineering microfluidic systems and biochemical protocols used routinely in the lab to provide simple and useful research tools (increasing assay throughput or reducing time from sample-to-result).