Cell culture in Acoustic Levitation

Present ollaborators : Jean-Michel Peyrin (DR CNRS), Marie-Pierre Junier (DR INSERM), Elias El-Habr (Pr.) at NeuroSciences Paris Seine, NPS, Sorbonne Université.

Shuake Kuermanbayi (PhD Student, co-supervised with MPJ), Litan Wang (PhD student, co-supervised with JMP), Guillaume Viraye (PhD Student co-sipervised with JMP) and Arthur Sureau (co-supervised with JMP)

Past collaborators : Nathan Jeger-Madiot (Postdoc), Chloé Dupuis (PhD student), Xavier Mousset (PhD student), Pierre-Ewen Lecoq in co-supervision with Jean-Michel Peyrin (Neurosciences Lab, Sorbonne Unviersity).

Lucile Rabiet (PhD Student), co-supervised with Jérôme Larghéro and Lousineh Arakélian (Cell Therapy Lab of Saint-Louis Hospital).

Athanasia Kritstakou, Andrea Coronel also collaborated with us at the very begining.

Acoustic Radiation Force (ARF) allows smooth manipulations of a large number of cells in suspension in a micro-cavity or a micro-channel.

We use ARF to design acoustic trap that allows long-terms cell culture in acoustic levitation in acoustic confinement.

We work on various cell types, either to study the cell evolution and behavior when cultured in acoustic levitation, away from the walls, or to design specific cell structures, from monolayers to organoïdes. The following figure shows a large aggregate of MSC (Mesenchymal Stem Cells) in acoustic levitation (collaboration with the Cell Therapy Lab of Jérôme Larghéro in Saint-Louis Hospital).

Formation of spheroids of neurons in acoustic levitation.