Workshop – Lipidomics: principles & applications in health in France (Bordeaux, Toulouse and Villeurbanne)

Combining theory and practical applications, this workshop aims to provide participants with an overview of the state of the art in lipidomics approaches to healthcare, their interests and limitations, and the challenges associated with setting up suitable workflows.

The registration deadline is 5 September 2025.

Registration here : https://ateliersinserm.dakini-pco.com/formulaire.d.inscription-61-48.php

The workshop will be organised in two phases:

• Phase I: Theory, from 1 to 3 December in Bordeaux.
• Phase II: Practice, two days in January in Toulouse and Villeurbanne.

It is organised by: Sophie AYCIRIEX (ISA, Villeurbanne), Justine BERTRAND-MICHEL (MTH-MetalToul, I2MC,Toulouse) & Emilie LAYRE (IPBS, Toulouse)

Phase I program :

EMBARKING ON THE LIPIDOMICS ROAD – Justine BERTRAND-MICHEL (I2MC, FRA), Sophie AYCIRIEX (CNRS UMR 5280, FRA), Laura GORACCI (University of Perugia, ITA) & Yu XIA (Tsinghua University, CHN)

MOLECULAR NETWORK – Pieter DORRESTEIN (University of California, USA)

INFLAMMATION AND METABOLIC DISEASES – Valerie O’DONNELL (Cardiff University, GBR) & Maria FEDOROVA (Center for Membrane Biochemistry & Lipid Research, DEU)

BIOMARKERS – Michal HOLCAPEK (University of Pardubice, CZE), Andrej SHEVCHENKO (Max Planck Institute of Molecular Cell Biology and Genetics, DEU) & Martin GIERA (Leiden
University, DEU)

LIPIDOMICS AND FLUXOMICS – Jean-Charles PORTAIS (Université Paul Sabatier, FRA) & Ondrej KUDA (IPHYS, CZE)

TOOLS FOR LIPIDOMICS RESULTS MEANING – Nathalie POUPIN (INRAE, FRA)

SPATIAL LIPIDOMICS – Maxence WISZTORSKI (Inserm U1192, FRA) & Martina MARCHETTI (TU Wien, AUT)

LIPIDS DISCOVERY – Emilie LAYRE (CNRS UMR 5089, FRA) & Nicolas CÉNAC (IRSD, FRA)

 

Phase II program :

Sample preparation, targeted & semi-targeted lipidomics (Toulouse): It will include preparation of samples for lipidomics in liquid-liquid extraction (biological fluids and tissues) and their analysis in GC-MS for fatty acid profiling and in LC-MS for semi-targeted profiling of phospholipids and major sphingolipids on low (QqQ) and high-resolution (Orbitrap) MS instruments.

Spatial lipidomics & Structural Characterization (Villeurbanne): It will include MALDI imaging analyses, from sample preparation (mouse liver or brain samples) to data processing. Same samples will be analyzed by untargeted analysis using a high-resolution mass spectrometer with ion mobility and alternative fragmentation modes (CID vs EAD).

SELECTION: 8 trainees for Toulouse and 4 trainees for Villeurbanne will be selected among Phase I participants.